Imagej quantification western blot3/31/2024 In such cases, a small peptide tag can be introduced, which is detectable with a tag-specific antibody. While commercial POI-specific antibodies are available for many proteins from model organisms and for well-studied targets, no suitable antibodies are on the market for the majority of proteins. ![]() In addition, quantitative analyses are complicated 4 and often result in a hyperbolic rather than in the desired linear signal-to-substrate relationship 1, 4, 7. Even with identical samples and standard operating procedures, results from different users may vary significantly 6. This impedes the comparability of different blots, in particular across different labs 3, 4, 5. Overall, the procedure involves multiple steps, which are often adapted depending on the target protein, the chosen antibody combination, or the sample composition 1, 2. After additional binding sites on this membrane have been blocked, target-specific antibodies are used to detect the protein of interest (POI), and these, in turn, are visualized by reporter-conjugated antibodies. Here, proteins within a complex mixture are separated on a polyacrylamide gel and transferred to a membrane. ![]() Western blots present one of the most widely used methods in molecular research.
0 Comments
Leave a Reply.AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |